DNA, for its intrinsic properties of high stability, biocompatibility and versatility, is an extremely suitable polymer to build nanostructures for biomedical applications.
We have recently shown that pristine octahedral DNA nanocages are stable in human serum and specifically and efficiently internalized by a receptor-mediated mechanism.
1) design and assemble DNA nanocages of different size and geometry;
2) functionalize them through orthogonal conjugation (click) chemistry with ligands and recombinant antibody fragments directed against the a-Folate Receptor (aFR);
3) modify them with different degree of poly ethylene glycol to eliminate nonspecific adsorption of serum proteins and greatly increase their resistance against degradation;
4) in vitro characterize their binding and internalization properties using a panel of cancer cell lines overexpressing the aFR;
5) follow, through imaging, the trafficking of the functionalized DNA nanocages in different mouse model systems;
6) evaluate their possible immunogenicity.
Composition research project:
S. Biocca, University of Roma Tor Vergata
F. Couillaud, University of Bordeaux
M. Figini, Istituto Nazionale Tumori Milano
F. Albericio, Barcelona University
Research @ NAST Centre 27 February
Research date: February 2017