NEURO ConTE towards clinical applications

The Project NEURO ConTE – Self-grating carbon nanotubes on polymers for stretchable electronics

A group researchers from NAST Centre, led by P. Morales (ENEA Casaccia) and R. Senesi (University of Rome Tor Vergata) in collaboration with IRCCS Neuromed, Pozzilli, Italy, have successfully fabricated and tested elementary bidimensional circuitry made of single-wall carbon-nanotube–based conductors, self-grafted on different polymer films, for electrocorticograms in an attempt to develop a simple technology for flexible and stretchable electronic devices. Unlike in other studies of polymer-carbon nanotube composites, no chemical functionalization of single-wall carbon nanotubes is necessary for stable grafting onto several polymeric surfaces, suggesting viable and cheap fabrication technologies for stretchable microdevices.

As a first example of technological application, the electrical functionality of a carbon nanotube– based 6-sensor (electrode) grid was demonstrated by recording of subdural electrocorticograms in freely moving rats over approximately three months. The results are very promising and may serve as a basis for future work targeting clinical applications.



Fig. 1. SEM images of SWCNT layers on MD-PE at different angles show many nanotube bundles firmly bonding the conductive layer to the polymer surface. Panel (a) shows strained nanotubes on attempting detachment of the SWCNT layer from the polymer (note that some nanotubes are broken due to high tension). Panel (b) is taken, on a different specimen, at a very low angle, nearly parallel to the surface at the edge of the SWCNT layer and shows the large amount of binding nanotubes well rooted into the polymer. Both images were recorded after a few sticky tape strippings.





immagine2Fig. 2. Characterization of stretched SWCNT wire grafted on MD-PE. Panels (a) and (b): Severe stretching. Doubling the length of the polymer substrate produces a bellows like structure, as shown in (a), (b), that is still conductive. Panel (c): The SEM image also shows single SWCNT bundles bridging the majority of the cracks that form the extended bellows. Panel (d): The unstretched, suspended polymer film shows wrinkling due to SWCNT grafting, which disappears on slight stretching (e).

P. Morales, S. Moyanova, L. Pavone, L. Fazi, D. Mirabile Gattia, B. Rapone, A. Gaglione and R. Senesi
Eur. Phys. J. Plus (2018) 133: 214
DOI 10.1140/epjp/i2018-12040-0

Link Adnkronos

Functionalized DNA nanostructure for cancer treatment

Potential calculatedDNA, for its intrinsic properties of high stability, biocompatibility and versatility, is an extremely suitable polymer to build nanostructures for biomedical applications.
We have recently shown that pristine octahedral DNA nanocages are stable in human serum and specifically and efficiently internalized by a receptor-mediated mechanism.

1) design and assemble DNA nanocages of different size and geometry;
2) functionalize them through orthogonal conjugation (click) chemistry with ligands and recombinant antibody fragments directed against the a-Folate Receptor (aFR);
3) modify them with different degree of poly ethylene glycol to eliminate nonspecific adsorption of serum proteins and greatly increase their resistance against degradation;
4) in vitro characterize their binding and internalization properties using a panel of cancer cell lines overexpressing the aFR;
5) follow, through imaging, the trafficking of the functionalized DNA nanocages in different mouse model systems;
6) evaluate their possible immunogenicity.nanocage


Alessandro Desideri

Composition research project: 
S. Biocca, University of Roma Tor Vergata
F. Couillaud, University of Bordeaux
M. Figini, Istituto Nazionale Tumori Milano
F. Albericio, Barcelona University

Related Links 
Research @ NAST Centre 27 February

Research date: February 2017

Neutrons to unveil the secrets of Violins and Egyptian artifacts

Scientists from NAST Centre are now able to capture previously elusive details about ancient Italian violins, by Amati and Testore, and the Egyptian grave-goods of Kha thanks to a new instrument, IMAT, which has the ability to reveal hidden images of these objects for the first time. The study is performed within the ARKHA project.

IMAT was opened today in the presence of H. E. Pasquale Terracciano, Italian Ambassador in London, by Corrado Spinella, Director of the Department of Physics of the Italian National Research Council (the CNR) and ISIS Director Robert McGreevy, in a celebration of the long-standing partnership between the UK and Italy.

The new imaging capabilities of IMAT enable scientists to access key information on the effects that the choice of different materials in violin has on the sound they produce, as well as the methods used by ancient Egyptians to make vases and alabasters.

Egyptian Artefact mounted on the neutron beamline

Cultural heritage studies of this kind, along with a number of other research areas, have expanded as a result of the Italian collaboration with the ISIS Neutron and Muon Source, a research facility supported by the Science and Technology Facilities Council. The agreement between CNR and ISIS was originally signed in 1985, making Italy one of the major and longest-standing partners of the research facility.

Today, the relationship between ISIS and Italy was celebrated with the opening of IMAT, the first instrument at ISIS capable of producing 3-dimensional images to reveal the inner workings of components across a wide range of science and technology areas.

H. E. Pasquale Terracciano, Italian Ambassador in London said: “This is a flagship European partnership of which both UK and Italy can be proud. We look forward to the continued success of this partnership far into the future.”

Dr Corrado Spinella, Director of the Department of Physics of the Italian National Research Council (the CNR) said: “It is wonderful to mark this milestone in the relationship between ISIS and Italy. Continued access to ISIS will be a key element of the Italian neutron research infrastructure strategy.”

Professor Robert McGreevy, Director of ISIS added: “We are delighted to be able to celebrate the inauguration of IMAT with our colleagues from CNR and H. E. Terracciano. Our partnership with Italy has brought extensive benefits not just to our two countries but the wider scientific community. The studies taking place today will provide important insights into our cultural heritage. Longer term, we expect to see IMAT enable advances in a wide range of applications, from aerospace, civil engineering and power generation to earth science and agriculture.”

Antonio Stradivari (1644 – 1737) was one of the greatest luthiers of all time. His interpretation of geometry and design for the violin has served as a conceptual model for violin-makers for more than 300 years.

Today a team of scientists from the Italian National Research Council CNR, Tarisio (London), Fondazione Pro Canale (Milan), University of Milano-Bicocca and University of Rome Tor Vergata, are investigating the craftsmanship of ancient Italian violins using neutron beams. The team wishes to explore how the wood, only a few millimetres thick, was used to construct the instrument, and how small details in the finishing and varnishing processes influence the sound production. Due to their peculiar shape and high value, violins are difficult objects to study with standard analytical techniques. IMAT offers a non-destructive method by which the entire instrument can be imaged. These experiments form part of a wider project that aims for the integrated analysis of musical instruments.

More than 3,000 years before Stradivari’s time, between 1425 and 1353 BC, Kha was overseeing the building of tombs for the kings of ancient Egypt. Previously, all that was known about Kha was taken from his mud brick funerary chapel. Until 1906, when an Italian archaeological mission discovered the shaft of Kha’s own tomb that had been sealed by a landslide. It was the richest and most complete non-royal burial assemblage ever found. Kha’s grave goods included coffin tunics, beds, vases and prestigious luxury objects inscribed with royal names, as well as alabaster containers, ceramic vessels and jars for food, oil powders and perfumes, some of which still remain sealed with their contents unknown.

Now, a selection of the Egyptian artefacts taken from Kha’s tomb, including vases and alabasters, are being studied on IMAT as part of the ARKHA project. Using neutron beams, which have a high penetration power in metals, the team will find clues on the methods used by ancient Egyptians to make the bronze vases and pots, as well as the state of their conservation.

img_2814 img_2815




The neutron tomography of a sealed alabaster vessel from the Egyptian Museum of Turin reveals the elemental composition of its contents.



Christian Greco, Director of Museo Egizio said: On the 2nd of October, 11 vases from the funerary goods of the tomb of Kha have been sent to the ISIS laboratory where they will be studied with the help of neutron beams in order to discover what is hidden inside these precious objects. We strongly believe in the collaboration between archaeologists and researchers in Chemistry and Physics that this project aims at promoting. It is an especially important initiative this year which marks the 110th anniversary of the discovery of the tomb of Kha by Ernesto Schiaparelli, dating back to 1906, because it represents an opportunity to offer to the public new data and useful new results about the objects belonging to our collection”.

img_1990-e1476396233971-500x500  Amati Violin monted on IMAT ready to be scanned

Carlo Chiesa from Fondazione Pro Canale, a Violin maker who acted as a consultant for the project, said: “New analytical methods will help us understand and rediscover techniques that three or four Century ago were a common knowledge in the small community of cremonese violin makers and allowed them to produce the small number of violins that are still unsurpassed for their acoustical properties.”

Prof Carla Andreani, who coordinates the Italian team on behalf of University Rome Tor Vergata added: “The two violins by Amati and Testore became the first ever stringed instruments to be scanned by a neutron beam at ISIS. In a few months, with the combined images produced with the contribution of all the partners of the project, we will be able to complete the elemental analysis using neutron resonances at higher energies and tomography and using thermal neutrons, and provide more complete information on inner structure and morphology of the Violins.”

Immagine_ufficio stampa

Researchers of CNR, NAST Centre, Egyptian Museum, “Archaeology Superintendent, fine arts and landscape for the metropolotana city of Turin”, Historical Museum of Physics and Centre for Studies and Research “Enrico Fermi”, University of Milan Bicocca, University of Cosenza

Prof. Giuseppe Gorini from University of Milano Bicocca said: “The collaboration allowed the temporary exportation of the objects from Italy to be analyzed at the ISIS neutron source. Restoration experts – from the Museo Egizio in Turin and Soprintendenza Archeologia del Piemonte – and University researchers worked closely, providing a positive example of multidisciplinary collaboration.”

Further Information

ARKHA (ARchaeology of the invisible: unveiling the grave goods of KHA) project. Objective of the study is to characterize the “Kha and Merit collection”, by the integrated use of non invasive and non destructive chemical and physical techniques by the integrated use of light and neutron probes. ARKHA combines competences of scientists belonging to different disciplinary fields, i.e curators, restorations, Egyptologists, archeologists, chemists and physicists.

ARKHA project is a collaboration partnership among:
Università degli Studi di Roma Tor Vergata
Consiglio Nazionale delle Ricerche
Museo Egizio di Torino
Museo Storico della Fisica e Centro Studi e Ricerche “Enrico Fermi”
Soprintendenza Archeologia, Belle Arti e Paesaggio per la Città Metropolitana di Torino
Università degli Studi di Milano-Bicocca

IMAT details
The IMAT beamline is a new instrument at the Science Technology Facilities Council’s ISIS Neutron and Muon Source. It will be the first instrument at ISIS capable of producing 3-dimensional images, revealing the inner workings of components across a wide range of science and technology areas.

The images produced by IMAT will have applications in the areas of aerospace and transportation, civil engineering, power generation, earth sciences, cultural heritage and agriculture. As neutrons can penetrate deeply into materials, IMAT will be capable of producing images of the internal structure of large engineering components. It will reveal, for example, the passage of lubricants and fuel through engine components, and the internal stresses and strains inside welded components.

IMAT has been built using funds provided by the UK government, together with imaging systems developed by Italian scientists from CNR-IPCF, funded through the Italian CNR research council.

The IMAT blockhouse

University of Rome Tor Vergata
ISIS Neutron and Muon Source
Centro Fermi
Tarisio London
Egyptian Museum in Turin
University Milano Bicocca

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UK lab helps reveal secrets of 3000 year old Egyptian vases – STFC News

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Research date: October 2016

The soft confinement of water in Graphene Oxide Membranes

Researchers from NAST Centre have studied the single particle hydrogen dynamics in 2D Graphene-Oxide at the ISIS Spallation Neutron source.

This 2D material has gained an increased interest in the last few years, given the presence of polar functional groups on Graphene-Oxide layers makes the material hydrophilic, thus favoring large quantities of water to be adsorbed between layers.

Water confined in GO has been characterized using the Vesuvio spectrometer at ISIS Neutron and Muon Pulsed Source in UK. The hydrogen local chemical environment under spatial confinement is discussed in the context of the interaction between the interfacial water and ice and the confining hydrophilic surface of the GO sponge. Mass-resolved neutron spectroscopy allowed a simultaneous and non-destructive measurement of water-uptake levels in hydrated and dried samples and highlight the effect of confinement on the hydrogen local potential. Hydration of the Graphene-Oxide Membranes was also extensively characterised by X-ray diffraction, atomic-force microscopy, thermal gravimetric analysis and elemental analysis by dynamic flash combustion.

Results reveale that hydrogen dynamics is little affected by the extreme confinements within graphene-oxide layers, i.e. the number of structural hydrogens from hydroxyl groups is negligible as compared to the one belonging to water, trapped during the production process.


Figure 1 Pictorial reconstruction of water confined between bi-dimensional layers of Graphene-Oxide.

figura 2figura 3

Joint collaboration:

NAST Centre,


Further Information

For more information please contact G. Romanelli (, and Roberto Senesi (email:


Further reading

Physical Chemistry Chemical Physics

Research date: June 2016

X-ray-phase-contrast-tomography brings back 2000-year-old “voice” of Philodemus


Researchers from NAST Centre  discovered the largest portion of Philodemus Greek text ever detected so far inside unopened carbonized Herculaneum papyri scrolls, through a virtual unrolling and deciphering using enhanced X-ray phase-contrast tomography. Figure 1 (top) shows a 3D tomographic reconstruction of the rolled carbonized Herculaneum papyri discovered in the Roman ‘Villa dei Papiri’ at Herculaneum in the middle of 18th century.

Ancient Herculaneum-papyrus scrolls, hopelessly charred in the 79 A.D. Vesuvius eruption, contain valuable writings of the Greek philosophers of the day, including thoughts of the Epicurean Philosopher Philodemus. X-ray-phase-contrast-tomography had recently begun unlocking their secrets.

Aim of this study was to read the text hidden inside carbonized-Herculaneum-papyri, through non-destructive ‘virtual-unrolling’, using enhanced X-ray-phase-contrast-tomography and dedicated algorithms.

Results of the study revealed the largest writing of Greek texts ever detected inside unopened scrolls, with unprecedented spatial resolution and contrast.

Texts have been unequivocally decoded and, for the first time, the “voice” of Epicurean philosopher Philodemus was brought back after 2000 years.

figura 3



Figure 2. Axial view of the 3D reconstruction revealing the complex stress field of the inner part of the scroll.






Figure 3. Fragments of carbonized Herculaneum papyri





Further Information

The interdisciplinary team included researcher from NAST Centre, CNR-NanoTec (coordinator), European Synchrotron Radiation Facility, Museo Storico della Fisica e Centro Studi e Ricerche Enrico Fermi, Università della Calabria, Interdisciplinary Center B. Segre Accademia Nazionale dei Lincei applied X-ray tomography to the study of rolled carbonized Herculaneum papyri’


I. Bukreeva, A. Mittone, A. Bravin, G. Festa, M. Alessandrelli, P. Coan, V. Formoso, R. G. Agostino, M. Giocondo, F. Ciuchi, M. Fratini, L. Massimi, A. Lamarra, C. Andreani, R. Bartolino, G. Gigli, G. Ranocchia, A. Cedola, ‘Enhanced X-ray-phase-contrast-tomography brings new clarity to the 2000-year-old “voiceof Epicurean philosopher Philodemus’, Scientific Reports 6:27227 | DOI: 10.1038/srep27227 (2016)

Related Links

University of Tor Vergata

Research date: July 2016



Light probes to unveil painting practices used in ancient Egypt

A large collection of artifacts from the Egyptian Museum of Turin was studied for the first time using analytical techniques. The study is performed within the ARKHA project. The collection is part of objects coming from ­one of the tombs discovered by Ernesto Schiapparelli’s Italian archaeological mission. In 1906 the archaeologist discovered the shaft of an underground tomb, containing the intact burial assemblage of two upper-class individuals, ‘the director of works’ Kha and his wife Merit. It was the richest and most complete non-royal burial assemblage ever discovered. Grave-goods include coffins tunics, beds, vases, cosmetics and prestigious luxury objects inscribed with royal names, which allowed to date his career between the reigns of Amenhotep II and Amenhotep III (1425-1353 BC). The grave-goods of Kha also include alabaster containers, ceramic vessels and jars for food, oil, powders and precious perfumes.

Painted caskets were studied by X-ray Fluorescence imaging. Results provide new clues on Egyptian painting techniques and the state of conservation of these ancient objects

Further Information

Researchers from NAST Centre applied for the first time non-invasive macro scan X-ray Fluorescence imaging (MA-XRF) to the Kha artifacts. The NAST Centre led an experimental team which included researchers from CNR Istituto per i Beni Archeologici e Monumentali (IBAM) of Catania and Istituto per i Processi Chimico Fisici (IPCF) of Messina, Museo Storico della Fisica e Centro Studi e Ricerche Enrico Fermi, the Museo delle Antichità Egizie di Torino and Soprintendenza Archeologia del Piemonte, Università degli Studi di Milano-Bicocca. The experimental apparatus was developed within a collaboration with CNR-IBAM and LANDIS laboratory of INFN-LNS. The new scanning methodology provided high-resolution images of the elemental distribution on the investigated paintings.

Preliminary results from the XRF scans of the Egyptian painted caskets coming for the Kha tomb already provided precious indications about the painting techniques applied, presence of different hands and their conservation status. The measurement campaign was performed in June 2016 at the Museo Egizio di Torino in the framework of a wider project aiming at the characterization of the intact Kha burial assemblage.

ARKHA (ARchaeology of the invisible: unveiling the grave goods of KHA) project. Objective of the study is to characterize the “Kha and Merit collection” by the integrated use of non invasive and non destructive chemical and physical techniques by the integrated use of light and neutron probes. ARKHA combines competences of scientists belonging to different disciplinary fields, i.e curators, restorations, Egyptologists, archeologists, chemists and physicists.

ARKHA project is a collaboration partnership among:

Università degli Studi di Roma Tor Vergata

Consiglio Nazionale delle Ricerche

Museo Egizio di Torino

Museo Storico della Fisica e Centro Studi e Ricerche “Enrico Fermi”

Soprintendenza Archeologia, Belle Arti e Paesaggio per la Città Metropolitana di Torino

Università degli Studi di Milano-Bicocca


Dr. Giulia Festa
Senior researcher at NAST Centre
University of Rome Tor Vergata,

Related Links and Media

University of Tor Vergata
CNR Press Note
Media Gallery

Research date: July 2016 

Evolution of hydrogen dynamics in amorphous ice with density

highlights_evolution-of-hydrogen_particIn stark contrast to the crystalline state, amorphous material display a complete lank of long range structural order. In the case of ice it came as a huge surprise that several different amorphous ices exist and they have been classified according to their densities. The waters molecules in ice are held together through hydrogen bonds and we have set aut to investigate how changes in density affect hydrogen bonding and nuclear quantum effects of the hydrogen atoms.

Date from the Vesuvio instruments showed a pronounced softening of the O-H stretching potential in the low-density ice compared with the higher- density ices. Moreover, the hydrogen mean kinetic energy was found to increase with increasing density indicating the weakening of hydrogen bonds as well as steeper and more harmonic hydrogen vibrational potential energy surface. In a novel approach, we used data from both the Vesuvio and Maria instruments to determine the anharmonicity constants of the O-H stretching modes. We then arrived at a very simple conclusion : hydrogen banding is the main cause for anharmonicity in amorphous ice.

Evolution :A. Parmentier ( Università degli Studi di Roma Tor Vergata, Italy), JJ Shephard ( University College London and Durham University), G. Romanelli ( Università degli Studi di Roma Tor Vergata, Italy, and ISIS), R. Senesi (Università degli Studi di Roma Tor Vergata and CNR-IPCF sezione di Messina, Italy), C.G. Salzmann (University College London) C. Andreani ( Universita degli Studi di Roma Tor Vergata, Italy).

Vesuvio, Mari.

Research Support:
Royal Society ( CGS, UF 100144); Leverhulme Trust ( RPG – 2014 – 04);
CNRISIS Agreement (2014-2020)

Dr. G. Romanelli,
Dr. C.G. Salzmann ,

Further information:
A. Parmentier et al. J. Phys. Chem. Lett., 6 (2015) 2038-2042



Non destructive neutron diffraction measurements of Ghiberti’s relief

Researchers from Center NAST studied two reliefs of Lorenzo Ghiberti from the ‘Gates of Paradise’ of the Battistero of Florence.

ghiberti3The study used neutron beams at the ISIS Spallation Neutron Source (UK) and at Research Neutron Source Heinz Maier-Leibnitz (FRMII) reactor (D). Results provide insight into the manufacturing techniques, alloys composition and the restoration techniques used, for the cleaning procedures i.e. laser polishing and immersion in the Rochelle salts. The investigation revealed for the first time that Ghiberti refused twice one of the gilded bronze relieves. Most likely this was a necessary action Ghiberti had to undertake for the first fusion presented defects.


Further Information
This study were performed in the framework of the Project ‘Ancient Charm’ a NEST (New and Emerging Science and Technology), within the Sixth Framework Programme (FP6); January 2006 – December 2009, supported by European Community.

Ancient Charm Partners
University of Milano-Bicocca (Coordinator), University of Roma Tor Vergata, Hugarian National Museum, Institute for Isotopes- Hungarian Academy of Sciences, Rheinische Friedrich-Wilhelms Universität zu Bonn (Germany), Universität zu Köln (Germany), European Commission – Joint Research Centre – Institute for Reference Materials and Measurements (Belgium), Leiden University (The Netherlands), Technical University of Delft (The Netherlands), Science and Technology Facilities Council (United Kingdom).

Figure 1. Researchers at ISIS spallation neutron source during the experimental campaign in April 2009.


Figure 2. Researchers at ISIS spallation neutron source during the experimental campaign in April 2009.


Figure 3. Researcher at ISIS spallation neutron source during the experimental campaign in April 2009.


Related Links

Journal of Applied Physics article (2011) 

Journal of Applied Physics article (2009)

University of Tor Vergata press release

TG Leonardo


Nova – Il sole 24 Ore


Direct Measurement of Competing Quantum Effects on the Kinetic Energy of Heavy Water upon Melting

iceberg_gio_0Even at room temperature, quantum mechanics plays a major role in determining the quantitative behavior of light nuclei, changing significantly the values of physical properties such as the heat capacity. However, other observables appear to be only weakly affected by nuclear quantum effects (NQEs); for instance, the melting temperatures of light and heavy water differ by less than 4 K. Recent theoretical work has attributed this to a competition between intra- and intermolecular NQEs, which can be separated by computing the anisotropy of the quantum kinetic energy tensor. The principal values of this tensor change in opposite directions when ice melts, leading to a very small net quantum mechanical effect on the melting point. This Letter presents the first direct experimental observation of this phenomenon, achieved by measuring the deuterium momentum distributions n(p) in heavy water and ice using deep inelastic neutron scattering (DINS) and resolving their anisotropy. Results from the experiments, supplemented by a theoretical analysis, show that the anisotropy of the quantum kinetic energy tensor can also be captured for heavier atoms such as oxygen. (The iceberg image in the Table of Contents and Abstract graphics was used with permission of the NOAA’s National Ocean Service, 2012


Carla Andreani, Roberto Senesi, Giovanni Romanelli – Dipartimento di Fisica e Centro NAST, Università degli Studi di Roma “Tor Vergata”, Via della Ricerca Scientifica 1, 00133 Roma, Italy

Michele Ceriotti, David E. Manolopoulos – Physical and Theoretical Chemistry Laboratory, University of Oxford, South Parks Road, Oxford OX1 3QZ, United Kingdom

Claudia Pantalei – Laboratoire Léon Brillouin, CEA, Saclay, France – Consiglio Nazionale delle Ricerche, CNR-IPCF, Sezione di Messina, Messina, Italy


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3D elemental mapping

Archaeological samples can be study via neutron resonances that can be used as fingerprints to identify and quantify elements within the bulk of the objects in a non-destructive and non-invasive manner. Neutron Resonance Transmission Imaging (NRTI) has been successfully applied to the 3D elemental mapping of archaeological samples.

A dedicated set-up at the INES (Italian Neutron Experimental Station) beamline of the ISIS spallation neutron source was used for the experiments. The archaeological sample is an early mediaeval disk fibula coming from the Hungarian National Museum in Budapest.

RT_NV_N76_1_45_Nr007 RT_NV_N76_1_45_Nr012

Frontal and lateral pictures of the analysed disc fibula, from Kölked-Feketekapu (Hungary), dated to the end of 6th century AD


Experimental hall of the ISIS spallation neutron source.


Further Information

Three-dimensional (3D) elemental imaging reconstruction of an ancient artefact by Neutron Resonance Transmission (NRT) was performed for the first time. NRT is a neutron technique based on the presence of a resonance structure in the neutron-induced reaction cross sections. These resonances allow the identification of elements and isotopes within the sample. A dedicated set-up on INES (Italian Neutron Experimental Station) beamline of the ISIS spallation neutron source was used for the experiments. The investigated artefact was an early mediaeval disc fibula from the Hungarian National Museum in Budapest, Hungary.


Dr. Giulia Festa,
Senior researcher at NAST Centre
University of Rome Tor Vergata


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Coupling DNA Nano-Breadboards to solid state conductors

Recent progresses in DNA nanotechnology have allowed the accomplishment of an almost infinite variety of nanosized objects of the most appealing shapes, endowed with hundreds of precisely located specific reaction sites. Can these DNA objects be exploited as ordering scaffolds to selforganize matter at the nano-scale? It was proven that different protein molecules can be arranged at specific positions on DNA scaffolds in solution, but then the problem arises of how to arrange and connect the nanoscaffolds on a wider, usable substrate, again in an ordered and addressable fashion.

A collaborative work between Centro NAST, ENEA, the cDNA Center of the University of Aarhus and the Center for Nanoscale Materials Science of the Oak Ridge National Laboratory, attempted the connection of such DNA nanoscaffolds to inorganic conductors (gold) orderly organized on a silicon dioxide surface; this aims at exploiting their potential as “nano-breadboards” for selfassembly of organic components and inorganic nanoparticles on them.


The team chose the simplest geometry of DNA nano-breadboard, a flat rectangular shape of approximately 75nm x 85nm, as thick as the DNA double helix, 2 nm. Even this simple and small board allows for bonding of more than 200 different components per face, on different specific locations on the boards, each determined within approximately 30 nm2. DNA boards are selfassembled from a long single stranded DNA represented by the M13 bacteriophage genome and 224 different oligonucleotides having selected sequences that hybridize different parts of the backbone and “staple” them together in the desired shape. This is the so called “DNA origami” technique. The staples at the corners of the rectangle are extended with sequences bearing two sulphur atoms that can later make covalent bonds to suitably arranged anchoring gold nanodots fabricated by electron beam lithography. And each of the stapling sequences can be extended with specifically designed sequences in order to bind the desired molecular components. Once the boards are assembled and purified they are allowed to react in solution with the gold nanodots, so that they can deposit and immobilize on them. In the first experiments, where groups of 4 nanodots at the corner of an 80 nm side square were used, the rate of correctly occupied nanodots was approximately 10%, which is satisfactory considering the statistical character of the origami-nanoanchor encounter, and their small size; nanodots are about 20 nm in diameter and 7-8 nm tall. Development of dielectrophoresis techniques to increase the rate of reaction is underway. Figure 1 shows AFM and SEM characterization of the gold nanodots, while Figure 2 shows an AFM topographic image of a DNA origami nanobreadboard docked and suspended onto its four gold nanodots. These will be later connected to an electrical detection system.


Authors from NAST Centre: Piero Morales, Mario Caruso, Liqian Wang, Antonio Rinaldi, Stefano Vespucci

Other Contributors: Kurt Gothelf, Mattia De Stefano, Abhichart Krissanaprasit, Jesper Vinther (Aarhus University); Claudia Dalmastri, Lucia Mosiello (ENEA), Scott Retterer (CNMS Oak Ridge Nat. Lab.)



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Monte Carlo Simulation of neutron techniques

The simulation group develops Monte Carlo simulations to study neutron related techniques. Simulations are used to study the feasibility of the neutron logging technique for cultural heritage applications, improve the neutron capture prompt gamma activation analysis, and investigate the sources of gamma ray background in pulsed neutron source facilities. The simulations are based on the well-estiblished Geant4 and ROOT codes.


Neutron Logging

Neutron-gamma logging is a nuclear technique based on measurements of gamma radiation emitted after excitation by a neutron source. The technique involves the use of a source of neutron radiation and a gamma detector lowered in a borehole. Neutrons produced by the neutron generator are slowed down through scattering with the nuclei of the material surrounding the borehole and are eventually absorbed. The gamma radiation yields information on the materials present in the irradiated volume and, in principle, can be used to sample unexcavated area and spot the presence of buried archeological artifacts. The technique has been successfully applied in mining industry and geology. We investigate the feasibility of the technique for applications in cultural heritage by using Monte Carlo techniques. A simulation model of a neutron logging system based on Geant4 has been developed to this aim.

Neutron capture prompt gamma activation analysis


Neutron capture prompt gamma activation analysis (PGAA) is a non-destructive analytical technique that provides information on the isotopic composition in samples. It based on the measurements of gamma-rays following neutron capture. The pattern of gamma-rays, ranging from hundred keV to 12 MeV, is unique for each isotope. The PGAA method has been applied in materials science, chemistry, geology, mining, archaeology, environment, food analysis, and medicine. A current limitation of the technique is gamma-ray self-absorption. Attenuation of gamma-rays within the sample itself that may lead to significant errors. We plan to exploit the gamma-ray self-absorption and investigate the feasibility of using low energies gamma-rays (< 1 MeV) to localize inclusions. Spatial distribution of elements has recently been measured by scanning a sample with a collimated neutron beam and by using a collimated gamma detector. Drawback of the technique is the high collimation of source and detector needed and the long beamtime required. Our method will make use of the full neutron beam area and allow for a fast localization of the inclusions. Information on position of the inclusion could be used to correct for the self-absorption and obtain more precise quantitative analysis. The studiy of the feasibility of the method and the optimization of the detectors’ geometry are carried out with Geant4 based Monte Carlo simulations.

Blockhouse background


Spallation neutron sources offer an unique opportunity for fundamental and applied science. The wide neutron spectrum produced from the spallation reaction ranges from meV to several MeV. At the ISIS pulsed neutron source, neutrons in the meV – eV region are employed, among other techniques, for diffraction and inelastic scattering. Neutrons in the eV-keV region are utilized for neutron imaging. High energy neutrons (MeV region) are used for electronic devices testing. One aspect to be considered in all applications is the gamma ray background. A clear estimation of the background and identification of the sources is of prime importance to increase the signal to noise ratio. We are developing a simulation tool based on Geant4 to investigate the sources of gamma radiation inside the blockhouse and reduce their contributions to the measurements.


Christopher Frost, PhD, ISIS
Giuseppe Gorini, PhD, University of Milano Bicocca


  1. A. Miceli, G. Festa, R. Senesi, G. Gorini, E. Perelli Cippo, R. Cattaneo, C. Andreani, “On-site Neutron Logging for Cultural Heritage Applications: a Monte Carlo Study’”, Submitted to Proceedings of the 17TH CHNT (2013)
  2. P. Schuetz , A. Miceli , I. Jerjen , A. Flisch, J. Hofmann, R. Broennimann, U. Sennhauser, Reducing environmental scattering in industrial computed tomography by system redesign, NDT & E International, (In press)
  3. P. Schuetz, A. Miceli, A. Flisch, I. Jerjen, J. Hofmann, U. Sennhauser, Strategies for the reduction of environmental scattering in X-ray Computed Tomography, Proceedings of Conference on Industrial Computed Tomography (2012)
  4. A Miceli, J Glister, A Andreyev, D Bryman, L Kurchaninov, P Lu, A Muennich, F Retiere and V Sossi, Simulations of a micro-PET system based on liquid xenon, Phys. Med. Biol. (2012)
  5. A. Berdondini, R. Brancaccio, V. D’Errico, A. Miceli, M. Bettuzzi, F. Casali, M. P. Morigi, M. Senn, A. Flisch,The Use of Industrial Computed Tomography in the Study of Archaeological Finds, 37th International Symposium on Archaeometry (2011)
  6. Pietropaolo, A., Cippo, E., Gorini, G., Tardocchi, M., Schooneveld, E., Andreani, C., et al., Gamma-Ray background sources in the VESUVIO spectrometer at ISIS spallation neutron source.Nucl Instrum Meth A (2009)
  7. A Pietropaolo, M Tardocchi, E Schooneveld, R Senesi, Characterization of the gamma background in epithermal neutron scattering measurements at pulsed neutron sources, Nucl Instrum Meth A (2006)

Biological electrochemical devices for energy production and storage

Collaborative research among University, public institutions and private enterprises for the development of biological Fuel Cells and Electrolysis Cells for the production of energy or Hydrogen.

Link to movie

Nanotechnology with Spider Silks

Spider fibers are an exceptionally resistant material based on the fibroin protein. The most obviuous property of spider fibers is however that they can be meters long but they are micro- or even nanometric in their cross section. This makes it relatively easy and inexpensive to manipulate the fibers and exploit their nanometric size properties. Furthermore, in spite of their sizes, due to their non uniform density, spider silks are efficient scatterers of visible radiation, which makes them easily observable with inexpensive equipment, contributing to the ease of manipulation. Fibers are often composed of fibrils that can be as thin as a few tens of nm, reaching thus the range of sizes of other natural exploitable nanoobjects such as rod type or filamentous and bacteriophage viruses.

After separating such fibrils from the bundle, The NAST Center team in collaboration with the ENEA Casaccia Surface Science laboratory used them for two simple nanotechnological applications: 1) as shadow masks to obtain extremely small and controllable nanogaps separating metal electrodes; 2) as a mechanical support to produce metallic micro- and nanowires.

Insulating gaps as narrow as 15 nm were obtained between gold electrodes over distances in the range of 10-3 or even 10-2 m. Gold was thermally evaporated on nanofibers, stretched across a slightly curved mica slab in order to obain a good adhesion; by rotation of the slab around an axis nearly corresponding to the adhering fiber, the designed shadow was made much smaller than the fiber itself.

Figure 1 shows an AFM topographic image of such electrodes obtained on stretching three fibers of


Figure 1. 600 nm, 250 nm and 15-20                                                       Figure 2 The electrical conduction
nm wide gaps ina gold layer obtained                                                      characteristics of 20 mm long
by shadowing with spider silks                                                                  gap vs those of 1 mm long gap, in air


different sizes, while figure 2 shows the lower insulating properties of a 2 cm long gap with respect to a 1 mm long gap.

Fibers were also gold coated uniformly all around by means of a suitable holder and their electrical conduction was probed. Figure 3 shows a linear, ohmic behaviour, which is maintained by slightly stretching the wire, while it drops to zero when stretched by more than 2% of it length.



Figure 3 . a) shows an SEM image of a double gold coated 350 nm
diameter spider fiber stretched by a nanomanipulated Scanning Probe
tip in the SEM vacuum chamber;

    1. b) shows the linear, ohmic behabviour of the current vs bias before the fiber breaks due to the stretching action





The ease of manipulation of spider fibers and the negligible cost of fabrication of nano-gaps and wires makes this method extremely useful for the straightforward assemblage and electrical connection of experimental microdevices, such as in the development and testing of lab-on-the-chip type 3D systems.

The full article can be accessed electronically at (Nanotechnology, Volume 23, Issue Number 25, 255304)

Authors from NAST Centre: Mario Caruso, Davide Flammini, Piero Morales

Other Contributors: Bruno Rapone and Piero Morales

Neutrons for Cultural Heritage studies

Collaborative research among scientists from Nast Centre, CNR , Università di Milano-Bicocca  and Cultural Heritage Institutions – Italian Ministry for Cultural Heritage, Opificio delle Pietre Dure , Museo Nazionale di Villa Giulia , Museo Civiche Raccolte Archeologiche e Numismatiche di Milano have investigated archaeological artefacts via integrated neutron techniques.

Neutron techniques are applied to cultural heritage artifacts to ‘unveil secrets’ in ancient objects. Measurements are devoted to study structural characteristics like composition, presence of alteration, crusts, inclusions, structure of the bulk, manufacturing techniques and presence of those elements which give us an overall fingerprint of the object’s characteristics. Neutrons penetrate through objects without substantial attenuation, a quality which makes them ideal for bulk, non-destructive testing.  Neutron Diffraction (ND)  is a method to study the crystal structure of the object. Components and their crystal structure can be detected. Neutron Tomography (NT) is a method of testing the internal structure of an object. A neutron beam impinging onto any heterogeneous object is differently transmitted depending on thickness, density, chemical composition and total cross section of the material along the line of sight. Recording the transmitted beam is possible to reconstruct the internal feature of the studied object. Prompt Gamma Activation Analysis (PGAA) and the newly Prompt Gamma Activation Imaging (PGAI) use thermal neutrons to detect and map the elemental composition of the irradiated samples. Neutron Resonance Capture Analysis (NRCA) use epithermal neutrons to detect elements and their isotopes in the irradiated sample. All these techniques are used as success applied to the study of cultural heritage objects. Following some studied cases.

Integrated neutron diffraction and imaging of Lorenzo Ghiberti’s relief from the Battistero di Firenze

Battistero di S. Giovanni, Florence

Battistero di S. Giovanni, Florence


This work is addressed to integrated neutron techniques for the study of an artifact, a gilded bronze relief, part of the most monumental Door, The Gates of Paradise, located at the Battistero di Firenze, masterpiece of the Florentine Renaissance by Lorenzo Ghiberti. The relief presents critical aspects regarding state of conservation and shows a re-melting whose extension and composition were unknown. The analysis was performed using Prompt Gamma Activation Imaging to reconstruct the elemental composition map, Neutron Diffraction and Radiography for an insight to manufacturing technique, and Neutron Resonance Transmission to map the gold layer distribution under the pollution deposits.

Prompt Gamma Activation Imaging (PGAI) and Neutron Radiographies
PGAI use thermal neutrons to map the elemental composition of the samples.
Prophet  Head contains: Cu, Au, Hg, Cl in the uncleaned part; Cu, Au, Hg in the area cleaned via Rochelle salts and Cu, Au, Hg and Cl in the area cleaned via laser ablation. Cl presence in the laser-cleaned area is relevant: Cl compounds represent a dangerous component as far as the corrosion processes is concerned.
High resolution neutron radiographies provided the extension and position of the re-melting area in the relief. This suggests that, because the original melting was not satisfactory, the sculptor made a second one.

Decorative element (Prophet Head) cleaned by a chemical solution and by laser

Decorative element (Prophet Head) cleaned by a chemical solution and by laser

Radiographies of the Prophet Head for 4 orientations

Radiographies of the Prophet Head for 4 orientations

Neutron Diffraction (ND) analysis

ND uses neutrons to study the crystal structure of the sample. In this case was devoted to measure cavities and inhomogeneity in the bulk of the sample, as well as bulk phase composition and residual strain distribution. Results:

1) re-melting was only a filler material without any particular treatment, primary melting suffered an homogenization process

2) a 3D map of the cavity created during second melting was created

3) it was demonstrated that the face and beard region were part of the second melting. Re-melting seems to have been realized by the artist to give thickness to the relief and make it much more hard-wearing.



1) G. Festa, C. Andreani, M. P. De Pascale, R. Senesi, G. Vitali, S. Porcinai, A. M. Giusti, R. Schulze, L. Canella, P. Kudejova, M. Mühlbauer, B. Schillinger and the Ancient Charm Collaboration, ‘A non destructive stratigraphic and radiographic neutron study of Lorenzo Ghiberti’s reliefs from Paradise and North doors of Florence Baptistery.’ Journal of Applied Physics, 106, N.4 (2009)

2) G. Festa, R. Senesi, M Alessandroni, C. Andreani, G. Vitali, S. Porcinai, A. M. Giusti, T. Materna, A. Paradowska, ‘Non destructive neutron diffraction measurements of cavities, inhomogeneities and residual strain in bronzes of Ghiberti’s relief from the Gates of Paradise’, Journal of Applied Physics in publication (2011)

The Prophet Head during the diffraction experiment

The Prophet Head during the diffraction experiment

Composition and corrosion phases of Etruscan Bronzes from Villanoviano Age

Etruscan bronzes coming from the Museo Nazionale di Villa Giulia, Rome, Italy were studied by Neutron Diffraction. The aim of the study was to collect information related to the main composition of the artefacts, the presence and nature of alterations or inclusions and the bulk structure to define the corrosion state, the past environments and the physical/chemical events that transformed the objects into partially corroded matrix. The experiments were carried out at ISIS spallation neutron source.

Etruscan bronze cup coming from Museo Nazionale di Villa Giulia and micrograph of sample surface

Etruscan bronze cup coming from Museo Nazionale di Villa Giulia and micrograph of sample surface

The bronze vases are part of an Etruscan collection discovered in the necropolises of Osteria-Poggio Mengarelli and Cavalupo in the outskirts of Vulci (Viterbo, Italy).

The samples, 14 fragments, were analysed with regard to chemical-phase compositions and corrosion products.

Bronze is composed of copper, tin (Cu, 85-95%, Sn, 5-15%) and small quantities of other metals. The determination of the exact copper/tin/lead relative composition provides important information on the working process and technologies adopted and, indirectly, on the authenticity of the artefacts. Results obtained from ND investigation of objects show that 60% to 95% by weight is un-corroded alloy that presents a composition (Cu = 90±4%) which is typical of low-tin ancient bronzes thus supporting the authenticity of the samples. ND measurements also provided information about working methods of bronzes through the analysis of relative bronze peaks width Dd/d as a function of d-spacing:  annealing and two different types of cooling dynamics (fast and slow) are present while hammering could also be present.  Furthermore data analysis showed that the combination of corrosion products in the single samples is in agreement with possible environmental conditions in which the objects were in contact. Most of the samples were buried. Some of these were in contact with salted water. This could come from seawater percolation or simply salted food contained in the vases. Additionally it has been possible to trace probable quantitative values for the concentration of certain environment components such as CO2 and NaCl present in the tombs.

Example of final GSAS Rietveld refinements plot

Example of final GSAS Rietveld refinements plot

Relative variation of the bronze-Bragg peak width for the 14 samples.

Relative variation of the bronze-Bragg peak width for the 14 samples


G. Festa, P. A. Caroppi, A. Filabozzi, C. Andreani, M. L. Arancio, R. Triolo, F. Lo Celso, V. Benfante, S. Imberti ‘Composition and corrosion phases of Etruscan Bronzes from Villanovan Age’,  Measurements Science and Technology, 19, 034004 (2008).


Neutron Tomography of Etruscan vase

A neutron tomography study was performed at the Antares experimental station of the FRMII reactor (Garching, near Munich) on an Etruscan little vase preserved at the Civiche Raccolte Archeologiche e Numismatiche (Milan, Italy). This is and Aryballos vase of Proto-Corinthian style (6-7th BC) and comes from the necropolis of the Banditaccia, in Cerveteri. It is made in ceramic material (5.9 cm height), with a globular body and a decoration with large and horizontal brown bands. It has an original cap still sealed and intact and the content is unknown. Aim of the study was to non-invasively observe the internal features of a sealed vase.  The analysis shows that the content is a fine powder well-preserved.

Aryballos proto-corinthian vase from the necropolis of the Banditaccia, in Cerveteri.

Tomography and radiographies of Aryballos proto-corinthian vase performed at ANTARES station (FRM-II, Garching, D)

Neutron Tomography results of an Etruscan Vase ( Video)

Research team:

E. P. Cippo1, G. Gorini1, R. Cattaneo1, G. Festa1,2, C. Andreani2

1 Università di Milano-Bicocca, Milano, Italy
2 Dipartimento di Fisica, Università degli Studi di Roma Tor Vergata,  Roma, Italy


Neutron diffraction study on ancient silver coins

A neutron diffraction study was performed on a series of ancient silver coins belonging to the Strait of Messina area (264- 241 b.C. e 218-202 A.D.). The aim of the study was to evaluate authenticity and, accordingly, to bear out an hypothesis of historical forgery.

The discovery of an ancient artifact often raises a variety of questions such as the correct determination of its historical and cultural time-frame, the place and method of production, the choice of treatments and conditions for restoration and preservation. In this field, new perspectives open up with the use of neutron-based techniques.

Neutrons, in fact, show a high penetration power through thick coatings or probable corrosion layers present in the sample, and provide  information from the bulk rather than from surface areas.

The period in which silver coins where introduced in Roman era is not exactly known but it is reasonably dated in the period of the Punic Wars (first: 264 − 241 BC and second: 218 − 202 BC), series of three wars fought between Rome and Carthage. In that period a big amount of money became necessary to cover war-expenditures. During this century a very large number of ‘fakes’ coins were used. It is not clear if they were made by the Roman Republic or they are forger work. This phenomenon regards, in particular, silver coins. The ‘authentic’ and the ‘fakes’ coins can be tell apart by eye because the ‘fakes’ have a core by copper or lead and an external layer made in silver. All the samples are republican-roman money delivered by Roma’s mint.

Photograph of coins.

The objects under study have been kindly furnished, within a long collaboration with IPCF-CNR, from the foundation Piccolo Museo S. Paolo in Reggio Calabria (Italy), here a very valuable numismatic collection belonging to a period ranging  from the ancient Magna Grecia to nowadays.Diffraction measurements were used in the characterization of the quantitative composition of the bulk of the coins. All the elemental analysis measurements have been performed at the INES station (Rutherford Appleton Laboratory, UK) and a complete phases distribution is provided.

The analysis of the diffraction measurements on the ancient coins shows the difference in composition: one of these is composed by only silver and it can be hold as authentic, instead two other coins are composed by a nucleus of copper with a cuprite patina and a silver covering and are clearly fakes.

Research team:

F. Aliotta1, L. Bartoli2,  D. Castrizio3, G. Festa4, R. Ponterio1, G. Salvato1, C. Vasi1

1 CNR – Istituto Processi Chimico-Fisici, Salita Sperone Contrada Papardo 98158 Faro Superiore Messina, Italy
2 CNR – Istituto di Fisica Applicata ­ 50019 Sesto Fiorentino, Italy
3 Dipartimento di Scienze dell’Antichità, Polo Annunziata  Università degli Studi di Messina, Italy
4 Dipartimento di Fisica, Università degli Studi di Roma Tor Vergata,  Roma Italy

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